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dc.creatorDEGRANDI, Tiago Marafiga-
dc.creatorPITA, Sebastian-
dc.creatorPANZERA, Yanina-
dc.creatorOLIVEIRA, Edivaldo Herculano Correa de-
dc.creatorMARQUES, José Ribamar Felipe-
dc.creatorFIGUEIRÓ, Marivaldo Rodrigues-
dc.creatorMARQUES, Larissa Coelho-
dc.creatorVINADE, Lucia Helena do Canto-
dc.creatorGUNSKI, Ricardo José-
dc.creatorDEL VALLE GARNERO, Analía-
dc.date.accessioned2015-09-10T12:45:03Z-
dc.date.available2015-09-10T12:45:03Z-
dc.date.issued2014-06-
dc.identifier.citationDEGRANDI, Tiago Marafiga et al. Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed. Genetics and Molecular Biology, Ribeirão Preto, v. 37, n. 2, p. 375-380, jun. 2014. Disponível em: <http://www.scielo.br/pdf/gmb/v37n2/a09v37n2.pdf>. Acesso em: 25 ago. 2015. <http://dx.doi.org/10.1590/S1415-47572014000300009>.pt_BR
dc.identifier.issn1415-4757-
dc.identifier.urihttp://repositorio.ufpa.br/jspui/handle/2011/6870-
dc.description.abstractDomestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the “river buffaloes” with 2n = 50 and the “swamp buffaloes”, 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs (3p, 4p, 6, 21, 23, 24) in the river buffaloes, whereas the swamp buffaloes presented only five NOR-bearing pairs (4p, 6, 20, 22, 23). The F1 crossbreed had 11 chromosomes with active NORs, indicating expression of both parental chromosomes. FISH analysis confirmed the numerical divergence identified with Ag-NOR. This result is explained by the loss of the NOR located on chromosome 4p in the river buffalo, which is involved in the tandem fusion with chromosome 9 in this subspecies. A comparison with the ancestral cattle karyotype suggests that the NOR found on the 3p of the river buffalo may have originated from a duplication of ribosomal genes, resulting in the formation of new NOR sites in this subspecies.pt_BR
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dc.description.provenanceMade available in DSpace on 2015-09-10T12:45:03Z (GMT). No. of bitstreams: 2 license_rdf: 22190 bytes, checksum: 19e8a2b57ef43c09f4d7071d2153c97d (MD5) Artigo_KaryotypicEvolutionRibosomal.pdf: 1252639 bytes, checksum: e3d056307e8062473874e8c3aae8f89c (MD5) Previous issue date: 2014-06en
dc.language.isoengpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectBubalinospt_BR
dc.subjectBubalus bubalispt_BR
dc.subjectCitogenéticapt_BR
dc.subjectDNApt_BR
dc.titleKaryotypic evolution of ribosomal sites in buffalo subspecies and their crossbreedpt_BR
dc.typeArtigo de Periódicopt_BR
Appears in Collections:Artigos Científicos - FACIN/ICEN

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