Isolamento e avaliação antitumoral de aiapina em tumor ascítico de ehrlich

Abstract

Ayapana triplinervis is an Amazonian plant species, popularly known as japan, which has coumarins as important chemical components, having its presence in relation to their pharmacological effects. Raw extracts of species have shown important antitumor effect. Therefore, this study aimed to isolate an A. triplinervis coumarin to evaluate its antitumor effect on ehrlich ascites tumor. For this, extracts of different polarities were captured from aerial parts of the plant and performed in phytochemical prospecting of plant medicines and extracts used. The best profile extract for coumarins was selected for the relationship by Column Chromatography. The coumarin fraction was subjected to a relationship and an isolated substance was identified by chromatographic methods and spectral. The acute toxicity and antitumor effects of coumarin were evaluated in mice, attracting the evaluation of body weight and cell viability in animals, intraperitoneally with 10, 20 and 40 mg / kg of ayapina. Phytochemical assays showed the presence of saponins, steroids and triterpenes, anthraquinones, polyphenols and coumarins. The ethyl acetate extract was selected for the relationship, without qualifying 28 fractions. As fractions obtaining or better selection profile for coumarins, they were from Fr 12 to 20. As fractions were pooled and refracted for coumarin isolation. Through the analysis it was possible to confirm the isolation of coumarin and identify it as 6,7- (methylenedioxy) coumarin, known as ayapina. Coumarin was considered to have low potential in Mexico. Aiapine showed antitumor effect with intraperitoneal treatment in mice with ascending tumor at doses of 10, 20 and 40mg / kg, by reducing the weight of animals and cancer cells without ascites fluid. Thus, the present study demonstrates for the first time the potential antitumor effect of ayapina.